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Calling All Super Saints...NOT!: Learn to celebrate who you are today while striving for your personal best!

為了解決Dwell time ms的問題，作者Walker, Tee 這樣論述：

Calling All Super Saints....NOT Edgy & Inspirational New Book Proves Christians Don't Have to be Perfect. Meticulously researched and written by Tee Walker, 'Calling All Super Saints...NOT ' quashes many misconceptions about the type of Christian God favors. While many believe that they need to be

perfect 'Super Saints', Walker instead argues that the only requirement of those who believe is to love themselves, whatever their circumstances in life. By sharing this powerful message, Walker hopes to take pressure off the Christian community and help those close to their faith focus on pro-activ

e service to the Lord, rather than dwelling on their shortcomings. Like millions of Christians around the world, Tee Walker struggled as her relationship with God developed. Often unclear about what was required of her and how she should act, Walker quickly became aware that trying to turn herself i

nto a 'Super Saint' was unrealistic and not required. In a bold effort to help others take the pressure of their relationship with the Lord and instead love themselves for who they really are, Walker is delighted to announce the release of her ground-breaking new book. 'Calling All Super Saints...NO

T ' calls on Christians to embrace themselves, their imperfections and to strive always to be the best person possible. "Using vignettes and applications from my own life, I am teaching others to love themselves, no matter who they are and no matter what their place in the world. This is what God re

ally wants - he doesn't want Super Saints. Many Christians need to wake up to this fact not only to kick-start their true service to God, but to become better and more content people in their own eyes," says Walker. Continuing, "I challenge each and every reader to acknowledge that they will never b

e perfect and to celebrate their daily progression toward being a better person. I've had many hiccups during my 'Christian Walk', and everyone else will; the key is not to dwell on them and to instead focus on all that really matters in life - God." Tee Walker is a wife and mother to 3 wonderful

boys currently residing in North Carolina. Ms. Walker has been writing most of her life. However, this is her first published literature based on personal experiences with God along with characters from the bible. Ms. Walker is an avid reader of Christian, mystery, and paranormal literature. Her oth

er interests is gardening and spending time with family and friends while sharing a meal. Ms. Walker earned her bachelor degree in Criminal Justice from Saint Leo University and is currently pursuing her Masters in Communication.

The Prairie

為了解決Dwell time ms的問題，作者Cooper, James Fenimore 這樣論述：

INTRODUCTION "The Prairie" was the third in order of Fenimore Cooper's Leatherstocking Tales. Its first appearance was in the year 1827. The idea of the story had suggested itself to him, we are told, before he had finished its immediate forerunner, "The Last of the Mohicans." He chose entirely new

scenes for it, "resolved to cross the Mississippi and wander over the desolate wastes of the remote Western prairies." He had been taking every chance that came of making a personal acquaintance with the Indian chiefs of the western tribes who were to be encountered about this period on their way in

the frequent Indian embassies to Washington. "He saw much to command his admiration," says Mrs. Cooper, "in these wild braves... It was a matter of course that in drawing Indian character he should dwell on the better traits of the picture, rather than on the coarser and more revolting though more

common points. Like West, he could see the Apollo in the young Mohawk." When in July, 1826, Cooper landed in England with his wife and family, he carried his Indian memories and associations with him. They crossed to France, and ascended the Seine by steamboat, and then settled for a time in Paris.

Of their quarters there in the Rue St. Maur, Sarah Fenimore Cooper writes: "It was thoroughly French in character. There was a short, narrow, gloomy lane or street, shut in between lofty dwelling houses, the lane often dark, always filthy, without sidewalks, a gutter running through the centre, over

which, suspended from a rope, hung a dim oil lamp or two-such was the Rue St. Maur, in the Faubourg St. Germain. It was a gloomy approach certainly. But a tall porte cochere opened, and suddenly the whole scene changed. Within those high walls, so forbidding in aspect, there lay charming gardens, g

ay with parterres of flowers, and shaded by noble trees, not only those belonging to the house itself, but those of other adjoining dwellings of the same character-one looked over park-like grounds covering some acres. The hotel itself, standing on the street, was old, and built on a grand scale; it

had been the home of a French ducal family in the time of Louis XIV. The rooms on the two lower floors were imposing and spacious; with ceilings of great height, gilded wainscoting and various quaint little medallion pictures of shepherds and shepherdesses, and other fancies of the time of Madame d

e Sevigne. Those little shepherds were supposed to have looked down upon la mere beaute, and upon la plus jolie fille de France as she danced her incomparable minuets. Those grand saloons were now devoted to the humble service of a school for young ladies. But on the third floor, to which one ascend

ed by a fine stone stairway, broad and easy, with elaborate iron railings, there was a more simple set of rooms, comfortably furnished, where the American family were pleasantly provided for, in a home of their own. Unwilling to separate from his children, who were placed at the school, the travelle

r adopted this plan that he might be near them. One of the rooms, overlooking the garden, and opening on a small terrace, became his study. He was soon at work. In his writing-desk lay some chapters of a new novel. The MS. had crossed the ocean with him, though but little had been added to its pages

during the wanderings of the English and French journeys."

Signal Processing in Stimulated Emission Based Pump-Probe Microscopy

為了解決Dwell time ms的問題，作者德士柏 這樣論述：

Fluorescence imaging has been a powerful technique due to its high sensitivity and specificity, especially for biomedical applications. Fluorescence emission is a spontaneous process and the photons are emitted incoherently and isotropically, filling full solid angle (4π). Therefore, high numerical

aperture (NA) optics and highly sensitive detectors are required for fluorescence imaging, to achieve both higher spatial resolution and efficient collection of the emitted photons.In comparison, stimulated emission (SE) exhibits a very different way of emitting and propagation, attributing to its

coherence. In SE based signal detection, two beams at pump-probe configuration are usually used, one at wavelength λ_pu (pump) and the other at λ_pr, (probe or SE). The pump beam is modulated and the modulation is transferred to the probe beam via stimulated emission of the excited fluorophores. Rec

ently, stimulated emission (SE) has presented as an advantageous method in fluorescence imaging due to its unique properties, including the detection of dark fluorophores and the integration with interferometric techniques.This doctoral thesis work focuses on three themes: first, the fundamental and

physical (or optical) limits of SE and its signal processing (Chapter 2). Second, the implement of high modulation frequency (subharmonic) for SE microscopy (Chapter 3) and third, demonstration of spontaneous loss (SL) detection (Chapter 4). In the first part, the development and the implement of S

E microscopy is realized with two synchronized semiconductor diode lasers. The SE signal is extracted by two different approaches of modulation, single and double modulation. The double modulation can extract SE signal by significantly improving the imaging contrast and removing spontaneous backgrou

nd. Rigorous theoretical estimation is also conducted by photon statistics observing Poisson distribution.The second part elucidates the implement of subharmonic fast gating synchronization for SE microscopy, to achieve much higher modulation frequency and better signal-to-noise ratio. The high modu

lation frequency (~38 MHz) of the pump laser is achieved with the frequency divider (FD) circuit on the probe laser’s repetition rate, which synchronously triggers the pump laser and provides the reference for the lock-in amplifier. The greatly improved signal-to-noise ratio allows close to shot noi

se limited sensitivity and reduced time constant (TC)~ 0.1 ms for the lock-in detection and pixel dwell time. The subharmonic modulation is also demonstrated on a labeled biological sample.The third part is aimed to demonstrate new methods. Note that dynamic range and detector saturation are the lim

iting factors for SE detection, as well as for other pump-probe contrasts. We have implemented spontaneous loss (SL) detection as a proof of concept in overcoming the above limiting factors. In this way, a high gain detector, such as a photomultiplier tube (PMT), can be selected for SE contrast. All

exemplification is conducted through ATTO 647N fluorescent dye.