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Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission

為了解決112 Cargo Tracking的問題，作者MOMOH GBETUWA 這樣論述：

Abstract Background: There has been great interest in identifying the biological substrate for light-cell interaction and their relations to cancer treatment. In our study, a single cell nuclear NIR (nNIR) and cytosol exposed NIR has been used to determine for the first-time mitochondria fragmentat

ion count (MFC) to compare NIR effect on subcellular cells. Near infra-red (NIR) possesses less light scattering and absorption in biological tissues it has a biological window that bears a very small photodamage and thus possesses a deep tissue penetration depth. Aim: To evaluate near-infrared (NIR

) laser focused into the nucleus (nNIR) or cytoplasm (cNIR) of a single living cell by a high numerical aperture condenser to dissect the novel role of cell nucleus in mediating NIR effects on mitochondrial dynamics of A549 non-small cell lung cancer cells.Materials and Methods: Cultured 150 single

cell of A549 nucleus and cytosol were incubated with 0.3 μM mitotracker green for 30 min washed with PBS, imaged control cell then treated with 224.02 J/cm2 NIR for 10 s and cells imaged at different time points of 1, 5, 10, 15 and 20 min. A549 cells were treated with conjugated 100 nM FND-EGF and i

ncubated with PD153035, caffeine and cetuximab for 1 h, and imaged cells. Results: Our analysis showed nNIR, but not cNIR, triggered mitochondrial fission in 10 minutes. On the contrast, the fission/fusion balance of mitochondria directly exposed to cNIR does not change. While the same phenomenon is

also triggered by single molecular interactions between epidermal growth factor (EGF) and its receptor EGFR, pharmacological studies with cetuximab, PD153035 and caffeine suggest EGF signaling crosstalk to DNA damaging response to mediate rapid mitochondrial fission as a result of nNIR irradiation.

These results suggest that nuclear DNA integrity is a novel biological target for cellular response to NIR. Conclusions: These results suggest that nuclear DNA integrity is a novel biological target for cellular response to NIR. Keywords: Keywords: near infrared (NIR), epidermal growth factor recep

tor (EGFR), mitochondrial fragmentation count (MFC), mitochondrial dynamic, cetuximab, caffeine.

應用第四方物流業者於我國個人電腦代工產業的貨物追蹤協作之關鍵因素探討:優化儲運系統

為了解決112 Cargo Tracking的問題，作者蔡政達 這樣論述：

在全球化競爭的環境下，一切講求速度，相對也考驗企業面對全球運籌時的應變能力，企業對於上下游協力廠商之間貨品的流通追蹤以及資訊的交互傳遞，能否達成彼此協同的境界，就成為產業的重要關鍵之一，我國台灣電腦代工產業貨品的全球運籌大都是以企業本身既有的獨立系統作業運作的，不論貨物是在企業的全球各地工廠貨倉間的運行，或是貨品直接出貨至全球交貨點位，現行貨物運籌的流程模式大都採委外的物流公司替企業承包貨物的載運業務，對其物流的資訊皆需藉由第三方物流公司獲取，故對於貨物資訊僅能仰賴間接取得、故一直存在著無法完全通透和一致性..等的問題。自近年因全球疫情開始至今邁入後疫情時代其全球的儲運情況因全球各地港口仍處

在持續性的塞港、缺船、缺貨櫃、…等問題中，這不僅對現有整個供應鏈體系的影響加鉅外而且更增加許多物流資訊的不確定性，以致供應鏈成員間無法及時獲取訊息的回應情況更加嚴重，長鞭效應的問題因而經常在供應鏈間發生。在本研究中是針對我國台灣電腦代工產業貨物在全球運籌供應鏈管理現況中所面臨的貨物追蹤及協作問題，提出以第四方專業物流業者(Fourth party Logistics, 4PLs)角色在供應鏈裡作為協同整合的功能概念，整理出其所具備的能力構面之關鍵因素，及應用 RFID(Radio Frequency Identification)，智能儲運系統的全球定位系統/通用封包無線服務GPS/GPRS(

Global Positioning System/ General Packet Radio Service)，雲端運算(Cloud Computing) 科技..等技術，建構出貨物追蹤資訊的協作流程概念平台，本論文中的研究方法採取德爾菲法的方式進行專家們對4Lls的物流業者所需具備的關鍵因素項目進行問卷調查探討，其結論針對供應鏈整合能力及資訊科技能力的兩項關鍵能力中的5大構面因素，30項準則項目，經兩次的重要性、共識性與一致性的意見整合過程，取得了26項重要準則項目，並以此重要項目為基礎，作為建構貨物追蹤資訊協作平台的成功關鍵項目的可行性依據，得以優化我國台灣電腦代工產業全球運儲管理的作業

系統。並藉此追蹤貨物協作系統模式，可提升供應鏈相關成員的產業競爭力，降低存貨風險，快速反應客戶端的需求。本研究的架構平台除協助我國電腦代工產業外，亦提供給其他電子產業，如屬產品週期短的科技相關產業，作為參考模式為本研究之主要貢獻。關鍵字：第三方物流公司、 無線射頻辯識技術、雲端運算、全球定位系統/通用封包無線服務、第四方專業物流業者、長鞭效應。